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Appl Environ Microbiol. 2012 Sep;78(17):6372-6. doi: 10.1128/AEM.00911-12. Epub 2012 Jun 22.

Differential in vivo gene expression of major Leptospira proteins in resistant or susceptible animal models.

Author information

1
Institut Pasteur de Nouvelle-Calédonie, Institut Pasteur International Network, Bacterial Research Laboratory, Noumea, New Caledonia.

Abstract

Transcripts of Leptospira 16S rRNA, FlaB, LigB, LipL21, LipL32, LipL36, LipL41, and OmpL37 were quantified in the blood of susceptible (hamsters) and resistant (mice) animal models of leptospirosis. We first validated adequate reference genes and then evaluated expression patterns in vivo compared to in vitro cultures. LipL32 expression was downregulated in vivo and differentially regulated in resistant and susceptible animals. FlaB expression was also repressed in mice but not in hamsters. In contrast, LigB and OmpL37 were upregulated in vivo. Thus, we demonstrated that a virulent strain of Leptospira differentially adapts its gene expression in the blood of infected animals.

PMID:
22729538
PMCID:
PMC3416592
DOI:
10.1128/AEM.00911-12
[Indexed for MEDLINE]
Free PMC Article

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