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Nat Neurosci. 2012 Jun 24;15(8):1102-4. doi: 10.1038/nn.3143.

Optogenetic silencing strategies differ in their effects on inhibitory synaptic transmission.

Author information

1
Department of Pharmacology, Oxford University, Oxford, UK.

Abstract

Optogenetic silencing using light-driven ion fluxes permits rapid and effective inhibition of neural activity. Using rodent hippocampal neurons, we found that silencing activity with a chloride pump can increase the probability of synaptically evoked spiking after photoactivation; this did not occur with a proton pump. This effect can be accounted for by changes to the GABA(A) receptor reversal potential and demonstrates an important difference between silencing strategies.

PMID:
22729174
PMCID:
PMC3428858
DOI:
10.1038/nn.3143
[Indexed for MEDLINE]
Free PMC Article

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