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FEBS Lett. 2012 Jul 30;586(16):2529-34. doi: 10.1016/j.febslet.2012.06.021. Epub 2012 Jun 21.

Persister-promoting bacterial toxin TisB produces anion-selective pores in planar lipid bilayers.

Author information

1
Program in Physical Biology, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA. gurnevp@mail.nih.gov

Abstract

We studied membrane activity of the bacterial peptide TisB involved in persister cell formation. TisB and its analogs form multi-state ion-conductive pores in planar lipid bilayers with all states displaying similar anionic selectivity. TisB analogs differing by ±1 elementary charges show corresponding changes in selectivity. Probing TisB pores with poly-(ethylene glycol)s reveals only restricted partitioning even for the smallest polymers, suggesting that the pores are characterized by a relatively small diameter. These findings allow us to suggest that TisB forms clusters of narrow pores that are essential for its mechanism of action.

PMID:
22728134
PMCID:
PMC3498054
DOI:
10.1016/j.febslet.2012.06.021
[Indexed for MEDLINE]
Free PMC Article

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