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Sci Rep. 2012;2:233. doi: 10.1038/srep00233. Epub 2012 Jan 25.

Impact of intestinal microbiota on intestinal luminal metabolome.

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1
Dairy Science and Technology Institute, Kyodo Milk Industry Co. Ltd., Tokyo 190-0182, Japan. m-matumoto@meito.co.jp

Abstract

Low-molecular-weight metabolites produced by intestinal microbiota play a direct role in health and disease. In this study, we analyzed the colonic luminal metabolome using capillary electrophoresis mass spectrometry with time-of-flight (CE-TOFMS) -a novel technique for analyzing and differentially displaying metabolic profiles- in order to clarify the metabolite profiles in the intestinal lumen. CE-TOFMS identified 179 metabolites from the colonic luminal metabolome and 48 metabolites were present in significantly higher concentrations and/or incidence in the germ-free (GF) mice than in the Ex-GF mice (p < 0.05), 77 metabolites were present in significantly lower concentrations and/or incidence in the GF mice than in the Ex-GF mice (p < 0.05), and 56 metabolites showed no differences in the concentration or incidence between GF and Ex-GF mice. These indicate that intestinal microbiota highly influenced the colonic luminal metabolome and a comprehensive understanding of intestinal luminal metabolome is critical for clarifying host-intestinal bacterial interactions.

PMID:
22724057
PMCID:
PMC3380406
DOI:
10.1038/srep00233
[Indexed for MEDLINE]
Free PMC Article
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