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BMC Microbiol. 2012 Jun 21;12:116. doi: 10.1186/1471-2180-12-116.

Transcriptomic profiling of Bacillus amyloliquefaciens FZB42 in response to maize root exudates.

Author information

1
Institute of Forest Protection, Nanjing Forestry University, Nanjing, China.

Abstract

BACKGROUND:

Plant root exudates have been shown to play an important role in mediating interactions between plant growth-promoting rhizobacteria (PGPR) and their host plants. Most investigations were performed on Gram-negative rhizobacteria, while much less is known about Gram-positive rhizobacteria. To elucidate early responses of PGPR to root exudates, we investigated changes in the transcriptome of a Gram-positive PGPR to plant root exudates.

RESULTS:

Bacillus amyloliquefaciens FZB42 is a well-studied Gram-positive PGPR. To obtain a comprehensive overview of FZB42 gene expression in response to maize root exudates, microarray experiments were performed. A total of 302 genes representing 8.2% of the FZB42 transcriptome showed significantly altered expression levels in the presence of root exudates. The majority of the genes (261) was up-regulated after incubation of FZB42 with root exudates, whereas only 41 genes were down-regulated. Several groups of the genes which were strongly induced by the root exudates are involved in metabolic pathways relating to nutrient utilization, bacterial chemotaxis and motility, and non-ribosomal synthesis of antimicrobial peptides and polyketides.

CONCLUSIONS:

Here we present a transcriptome analysis of the root-colonizing bacterium Bacillus amyloliquefaciens FZB42 in response to maize root exudates. The 302 genes identified as being differentially transcribed are proposed to be involved in interactions of Gram-positive bacteria with plants.

PMID:
22720735
PMCID:
PMC3438084
DOI:
10.1186/1471-2180-12-116
[Indexed for MEDLINE]
Free PMC Article

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