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PLoS Comput Biol. 2012;8(6):e1002519. doi: 10.1371/journal.pcbi.1002519. Epub 2012 Jun 14.

Visualization and analysis of 3D microscopic images.

Author information

1
Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, United States of America.

Abstract

In a wide range of biological studies, it is highly desirable to visualize and analyze three-dimensional (3D) microscopic images. In this primer, we first introduce several major methods for visualizing typical 3D images and related multi-scale, multi-time-point, multi-color data sets. Then, we discuss three key categories of image analysis tasks, namely segmentation, registration, and annotation. We demonstrate how to pipeline these visualization and analysis modules using examples of profiling the single-cell gene-expression of C. elegans and constructing a map of stereotyped neurite tracts in a fruit fly brain.

PMID:
22719236
PMCID:
PMC3375219
DOI:
10.1371/journal.pcbi.1002519
[Indexed for MEDLINE]
Free PMC Article

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