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Opt Express. 2012 Jun 18;20(13):14437-50. doi: 10.1364/OE.20.014437.

Quantitative 3D imaging of scattering media using structured illumination and computed tomography.

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Division of Combustion Physics, Lund Institute of Technology, Box 118, S-221 00 Lund University, Sweden.


An imaging technique capable of measuring the extinction coefficient in 3D is presented and demonstrated on various scattering media. The approach is able to suppress unwanted effects due to both multiple scattering and light extinction, which, in turbid situations, seriously hampers the performance of conventional imaging techniques. The main concept consists in illuminating the sample of interest with a light source that is spatially modulated in both the vertical and horizontal direction and to measure, using Structured Illumination, the correct transmission in 2D at several viewing angles. The sample is then reconstructed in 3D by means of a standard Computed Tomography algorithm. To create the adequate illumination, a novel "crossed" structured illumination approach is implemented. In this article, the accuracy and limitation of the method is first evaluated by probing several homogeneous milk solutions at various levels of turbidity. The unique possibility of visualizing an object hidden within such solutions is also demonstrated. Finally the method is applied on two different inhomogeneous scattering spray systems; one transient and one quasi-steady state.

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