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Methods Mol Biol. 2012;898:85-107. doi: 10.1007/978-1-61779-918-1_5.

Molecular tools for carotenogenesis analysis in the zygomycete Mucor circinelloides.

Author information

1
Departamento de Genética y Microbiología (Unidad Asociada al IQFR-CSIC), Facultad de Biología, Universidad de Murcia, Campus de Espinardo, Murcia, Spain. storres@um.es

Abstract

The carotene producer fungus Mucor circinelloides is the zygomycete more amenable to genetic manipulations by using molecular tools. Since the initial development of an effective procedure of genetic transformation, more than two decades ago, the availability of new molecular approaches such as gene replacement techniques and gene expression inactivation by RNA silencing, in addition to the sequencing of its genome, has made Mucor a valuable organism for the study of a number of processes. Here we describe in detail the main techniques and methods currently used to manipulate M. circinelloides, including transformation, gene replacement, gene silencing, RNAi, and immunoprecipitation.

PMID:
22711119
DOI:
10.1007/978-1-61779-918-1_5
[Indexed for MEDLINE]

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