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Int J Lab Hematol. 2012 Dec;34(6):584-93. doi: 10.1111/j.1751-553X.2012.01439.x. Epub 2012 Jun 18.

Comparison of the Cytodiff flow cytometric leucocyte differential count system with the Sysmex XE-2100 and Beckman Coulter UniCel DxH 800.

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1
Department of Laboratory Medicine, University of Ulsan and Asan Medical Center, Seoul, Korea.

Abstract

INTRODUCTION:

Routine automated haematology analysers categorize leucocytes into five types. The Cytodiff (Beckman Coulter) is a 16-part leucocyte differential analysis system that uses six markers and five colours. We compared leucocyte differential counts obtained by the Cytodiff with five-part differential counts obtained by routine automated haematology analysers.

METHODS:

We collected 477 EDTA blood samples from healthy individuals and patients with malignancies, sepsis and multi-organ failure. Leucocyte differential counts were simultaneously analysed by a Cytodiff multiparametric flow cytometric system and the XE-2100 (Sysmex) and UniCel DxH 800 (Beckman Coulter) automated haematology analysers. Regression and correlation analyses were performed between the different systems.

RESULTS:

Our Cytodiff results were well correlated with those produced using the DxH 800 and XE-2100 analysers except for monocytes and basophils. The correlations were poorer for leukopenic than for nonleukopenic samples. For most samples, Cytodiff obtained a higher correlation with manual counts according to a case analysis; however, in several samples, the Cytodiff generated false decreases in monocyte levels and false increases in basophil levels.

CONCLUSION:

The Cytodiff may have an advantage, as it could sensitively detect blasts and immature granulocytes. Additionally, it was less labour-intensive than manual counting, and therefore, the Cytodiff might be useful for differential counts.

KEYWORDS:

Cytodiff; DxH 800; XE‐2100; leucocyte differential count

[Indexed for MEDLINE]

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