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Langmuir. 2012 Jul 31;28(30):11265-73. doi: 10.1021/la301661x. Epub 2012 Jul 20.

Biofunctional paper via the covalent modification of cellulose.

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1
Department of Bioengineering, University of Washington, Seattle, Washington 98195, United States.

Abstract

Paper-based analytical devices are the subject of growing interest for the development of low-cost point-of-care diagnostics, environmental monitoring technologies, and research tools for limited-resource settings. However, there are limited chemistries available for the conjugation of biomolecules to cellulose for use in biomedical applications. Herein, divinyl sulfone (DVS) chemistry was demonstrated to immobilize small molecules, proteins, and DNA covalently onto the hydroxyl groups of cellulose membranes through nucleophilic addition. Assays on modified cellulose using protein-carbohydrate and protein-glycoprotein interactions as well as oligonucleotide hybridization showed that the membrane's bioactivity was specific, dose-dependent, and stable over a long period of time. The use of an inkjet printer to form patterns of biomolecules on DVS-activated cellulose illustrates the adaptability of the DVS functionalization technique to pattern sophisticated designs, with potential applications in cellulose-based lateral flow devices.

PMID:
22708701
PMCID:
PMC3449171
DOI:
10.1021/la301661x
[Indexed for MEDLINE]
Free PMC Article
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