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Sex Transm Dis. 2012 Jul;39(7):514-7. doi: 10.1097/OLQ.0b013e31824f2f5b.

Comparison of the BD Viper System with XTR Technology to the Gen-Probe APTIMA COMBO 2 Assay using the TIGRIS DTS system for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine specimens.

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1
Saskatchewan Disease Control Laboratory, Regina, Saskatchewan, Canada.

Abstract

BACKGROUND:

Performances of the BD ProbeTec Chlamydia trachomatis (CT)/Neisseria Gonorrhoeae (GC) Q(x) Amplified DNA Assay reagents on a BD Viper System with XTR Technology and APTIMA COMBO 2 Assay reagents on a TIGRIS DTS platform, for detection of both CT and GC were compared.

METHODS:

A total of 1018 first-void urine specimens were tested for the presence of CT and GC DNA using the 2 assays.

RESULTS:

CT was detected in 143 specimens (14%). Eight specimens exhibited discordant results, and they were divided equally between the 2 assays. Based on the original results, the overall agreement for CT was 99.2%, with 97.1% and 99.5% in agreement with positive and negative specimens, respectively. Cohen's Kappa was 0.967. GC was detected in 27 specimens (2.6%). Two specimens exhibited discordant results, and they were divided equally between the 2 assays. Based on the original results, the overall agreement was 99.8%, with 96.2% and 99.9% in agreement for positive and negative specimens, respectively. Cohen's Kappa was 0.961.

CONCLUSIONS:

There was a high level of agreement between the systems for both CT and GC detection.

PMID:
22706212
DOI:
10.1097/OLQ.0b013e31824f2f5b
[Indexed for MEDLINE]
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