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Nat Struct Mol Biol. 2012 Jun 17;19(7):719-21. doi: 10.1038/nsmb.2327.

Large-scale mapping of branchpoints in human pre-mRNA transcripts in vivo.

Author information

1
Laboratory of Molecular Medicine, Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, Rhode Island, USA.

Abstract

We present the first large-scale identification of lariats-the transient branched introns that are released as a byproduct of pre-mRNA splicing. The locations of the branchpoints in these introns provide insight into the early steps of splicing. From this data set, we have developed a comprehensive model of 3' splice-site selection, identified new mechanisms of alternative splicing and mapped the distribution of splicing factors around branchpoints.

PMID:
22705790
PMCID:
PMC3465671
DOI:
10.1038/nsmb.2327
[Indexed for MEDLINE]
Free PMC Article

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