Format

Send to

Choose Destination
See comment in PubMed Commons below
Cancer Cell. 2012 Jun 12;21(6):848-55. doi: 10.1016/j.ccr.2012.04.037.

Proliferation and tumorigenesis of a murine sarcoma cell line in the absence of DICER1.

Author information

1
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Abstract

MicroRNAs are a class of short ~22 nucleotide RNAs predicted to regulate nearly half of all protein coding genes, including many involved in basal cellular processes and organismal development. Although a global reduction in miRNAs is commonly observed in various human tumors, complete loss has not been documented, suggesting an essential function for miRNAs in tumorigenesis. Here we present the finding that transformed or immortalized Dicer1 null somatic cells can be isolated readily in vitro, maintain the characteristics of DICER1-expressing controls and remain stably proliferative. Furthermore, Dicer1 null cells from a sarcoma cell line, though depleted of miRNAs, are competent for tumor formation. Hence, miRNA levels in cancer may be maintained in vivo by a complex stabilizing selection in the intratumoral environment.

PMID:
22698408
PMCID:
PMC3385871
DOI:
10.1016/j.ccr.2012.04.037
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Support Center