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Nano Lett. 2012 Jul 11;12(7):3861-6. doi: 10.1021/nl302069q. Epub 2012 Jun 18.

Multicolor super-resolution DNA imaging for genetic analysis.

Author information

1
Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.

Erratum in

  • Nano Lett. 2013 Mar 13;13(3):1365.

Abstract

Many types of cancer and neurodegenerative diseases are caused by abnormalities and variations in the genome. We have designed a high-resolution imaging technique with high throughput and low cost for determining structural variations of genes related to genetic diseases. We initially mapped all seven nicking sites of Nb.BbvCI endonuclease enzyme on lambda DNA. Then we resolved densely labeled patterns of 107 nicking sites on human BAC DNA that is digested by Nb.BsmI and Nb.BbvCI endonuclease enzymes. This high density resulted in several dyes being closer together than the diffraction limit. Overall, detailed DNA nicking sites mapping with 100 bp resolution was achieved, which has the potential to reveal information about genetic variance and to facilitate medical diagnosis of several genetic diseases.

PMID:
22698062
PMCID:
PMC3880789
DOI:
10.1021/nl302069q
[Indexed for MEDLINE]
Free PMC Article
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