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ACS Chem Biol. 2012 Aug 17;7(8):1326-30. doi: 10.1021/cb300238w. Epub 2012 Jun 12.

State-selective metabolic labeling of cellular proteins.

Author information

1
Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 E. California Blvd., Pasadena, CA 91125, USA.

Abstract

Transcriptional activity from a specified promoter can provide a useful marker for the physiological state of a cell. Here we introduce a method for selective tagging of proteins made in cells in which specified promoters are active. Tagged proteins can be modified with affinity reagents for enrichment or with fluorescent dyes for visualization. The method allows state-selective analysis of the proteome, whereby proteins synthesized in predetermined physiological states can be identified. The approach is demonstrated by proteome-wide labeling of bacterial proteins upon activation of the P(BAD) promoter and the SoxRS regulon and provides a basis for analysis of more complex systems including spatially heterogeneous microbial cultures and biofilms.

PMID:
22692961
PMCID:
PMC3423470
DOI:
10.1021/cb300238w
[Indexed for MEDLINE]
Free PMC Article

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