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Mar Drugs. 2012 Apr;10(4):890-9. doi: 10.3390/md10040890. Epub 2012 Apr 10.

Astaxanthin treatment reduced oxidative induced pro-inflammatory cytokines secretion in U937: SHP-1 as a novel biological target.

Author information

1
Department of Medicine and Science of Aging, University G. D'Annunzio-Chieti, Chieti 66100, Italy. l.speranza@unich.it

Abstract

It has been suggested that oxidative stress activates various intracellular signaling pathways leading to secretion of a variety of pro-inflammatory cytokines and chemokines. SHP-1 is a protein tyrosine phosphatase (PTP) which acts as a negative regulator of immune cytokine signaling. However, intracellular hydrogen peroxide (H(2)O(2)), generated endogenously upon stimulation and exogenously from environmental oxidants, has been known to be involved in the process of intracellular signaling through inhibiting various PTPs, including SHP-1. In this study, we investigated the potential role of astaxanthin, an antioxidant marine carotenoid, in re-establishing SHP-1 negative regulation on pro-inflammatory cytokines secretion in U-937 cell line stimulated with oxidative stimulus. ELISA measurement suggested that ASTA treatment (10 µM) reduced pro-inflammatory cytokines secretion (IL-1β, IL-6 and TNF-α) induced through H(2)O(2), (100 µM). Furthermore, this property is elicited by restoration of basal SHP-1 protein expression level and reduced NF-κB (p65) nuclear expression, as showed by western blotting experiments.

KEYWORDS:

SHP-1 protein; astaxanthin; carotenoids; inflammation

PMID:
22690149
PMCID:
PMC3366681
DOI:
10.3390/md10040890
[Indexed for MEDLINE]
Free PMC Article

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