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Methods Mol Biol. 2012;884:353-61. doi: 10.1007/978-1-61779-848-1_25.

Determination of posttranslational modifications of photoreceptor differentiation factor NRL: focus on SUMOylation.

Author information

1
Neurobiology Neurodegeneration and Repair Laboratory, National Eye Institute, National Institutes of Health, Bethesda, MD, USA.

Abstract

Conjugation of SUMO (small ubiquitin-related modifier 1) is a critical posttranslational modification, with significant impact on protein function/activity. Here, we describe direct SUMOylation of GST (glutathione S-transferase)-fusion protein and immunoprecipitation assays for investigating SUMOylation of any protein of interest. We have employed these methods to examine SUMOylation of the basic-motif leucine zipper transcription factor NRL.

PMID:
22688719
DOI:
10.1007/978-1-61779-848-1_25
[Indexed for MEDLINE]

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