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Structure. 2012 Jun 6;20(6):967-76. doi: 10.1016/j.str.2012.04.010.

Fusion partner toolchest for the stabilization and crystallization of G protein-coupled receptors.

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1
Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.

Abstract

Structural studies of human G protein-coupled receptors (GPCRs) have recently been accelerated through the use of a fusion partner that was inserted into the third intracellular loop. Using chimeras of the human β(2)-adrenergic and human A(2A) adenosine receptors, we present the methodology and data for the initial selection of an expanded set of fusion partners for crystallizing GPCRs. In particular, use of the thermostabilized apocytochrome b(562)RIL as a fusion partner displays certain advantages over previously utilized fusion proteins, resulting in a significant improvement in stability and structure of GPCR-fusion constructs.

PMID:
22681902
PMCID:
PMC3375611
DOI:
10.1016/j.str.2012.04.010
[Indexed for MEDLINE]
Free PMC Article
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