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Am J Respir Crit Care Med. 2012 Aug 15;186(4):359-68. doi: 10.1164/rccm.201201-0057OC. Epub 2012 Jun 7.

A novel molecular signature for elevated tricuspid regurgitation velocity in sickle cell disease.

Author information

1
Institute for Personalized Respiratory Medicine,University of Illinois at Chicago, Chicago, IL 60612, USA.

Abstract

RATIONALE:

An increased tricuspid regurgitation jet velocity (TRV > 2.5 m/s) and pulmonary hypertension defined by right heart catheterization both independently confer increased mortality in sickle cell disease (SCD).

OBJECTIVES:

We explored the usefulness of peripheral blood mononuclear cell-derived gene signatures as biomarkers for an elevated TRV in SCD.

METHODS:

Twenty-seven patients with SCD underwent echocardiography and peripheral blood mononuclear cell isolation for expression profiling and 112 patients with SCD were genotyped for single-nucleotide polymorphisms.

MEASUREMENTS AND MAIN RESULTS:

Genome-wide gene and miRNA expression profiles were correlated against TRV, yielding 631 transcripts and 12 miRNAs. Support vector machine analysis identified a 10-gene signature including GALNT13 (encoding polypeptide N-acetylgalactosaminyltransferase 13) that discriminates patients with and without increased TRV with 100% accuracy. This finding was then validated in a cohort of patients with SCD without (n = 10) and with pulmonary hypertension (n = 10, 90% accuracy). Increased TRV-related miRNAs revealed strong in silico binding predictions of miR-301a to GALNT13 corroborated by microarray analyses demonstrating an inverse correlation between their expression. A genetic association study comparing patients with an elevated (n = 49) versus normal (n = 63) TRV revealed five significant single-nucleotide polymorphisms within GALNT13 (P < 0.005), four trans-acting (P < 2.1 × 10(-7)) and one cis-acting (P = 0.6 × 10(-4)) expression quantitative trait locus upstream of the adenosine-A2B receptor gene (ADORA2B).

CONCLUSIONS:

These studies validate the clinical usefulness of genomic signatures as potential biomarkers and highlight ADORA2B and GALNT13 as potential candidate genes in SCD-associated elevated TRV.

PMID:
22679008
PMCID:
PMC3443809
DOI:
10.1164/rccm.201201-0057OC
[Indexed for MEDLINE]
Free PMC Article

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