Format

Send to

Choose Destination
See comment in PubMed Commons below
Nat Methods. 2012 May 30;9(6):567-74. doi: 10.1038/nmeth.2043.

Counting stem cells: methodological constraints.

Author information

1
Laboratory of Stem Cell Research, European Research Institute for the Biology of Ageing, University Medical Center Groningen, University of Groningen, The Netherlands. l.bystrykh@med.umcg.nl

Abstract

The number of stem cells contributing to hematopoiesis has been a matter of debate. Many studies use retroviral tagging of stem cells to measure clonal contribution. Here we argue that methodological factors can impact such clonal analyses. Whereas early studies had low resolution, leading to underestimation, recent methods may result in an overestimation of stem-cell counts. We discuss how restriction enzyme choice, PCR bias, high-throughput sequencing depth and tagging method could affect the conclusions of clonal studies.

PMID:
22669654
DOI:
10.1038/nmeth.2043
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group
    Loading ...
    Support Center