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Mol Gen Genet. 1990 Dec;224(3):482-6.

Cloning and characterization of the Azotobacter vinelandii recA gene and construction of a recA deletion mutant.

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Genetic Engineering Unit, Jawaharlal Nehru University, New Delhi, India.


The recA gene of Azotobacter vinelandii was isolated from a genomic library by heterologous complementation of an Escherichia coli recA mutation for resistance to UV radiation. The A. vinelandii recA gene was localized on adjacent PstI fragments of 1.3 and 1.7 kb. The cloned A. vinelandii recA gene was functionally analogous to the E. coli recA gene. It was also able to complement the E. coli recA mutation for homologous recombination. A recA deletion mutant of A. vinelandii was constructed. This mutant was sensitive to DNA-damaging agents like UV rays, methyl methane sulfonate (MMS) and nalidixic acid and was deficient in homologous recombination.

[Indexed for MEDLINE]

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