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Biotechniques. 2012 Jun;52(6):381-5. doi: 10.2144/0000113878.

Trehalose-enhanced isolation of neuronal sub-types from adult mouse brain.

Author information

1
Omics Science Center, RIKEN Yokohama Institute, 1-7-22, Suehiro Cho, 230-0045, Tsurumi, Yokohama 230-0045, Japan.

Abstract

Efficient isolation of specific, intact, living neurons from the adult brain is problematic due to the complex nature of the extracellular matrix consolidating the neuronal network. Here, we present significant improvements to the protocol for isolation of pure populations of neurons from mature postnatal mouse brain using fluorescence activated cell sorting (FACS). The 10-fold increase in cell yield enables cell-specific transcriptome analysis by protocols such as nanoCAGE and RNA seq.

PMID:
22668417
PMCID:
PMC3696583
DOI:
10.2144/0000113878
[Indexed for MEDLINE]
Free PMC Article

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