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Nano Lett. 2012 Jul 11;12(7):3690-4. doi: 10.1021/nl301480h. Epub 2012 Jun 8.

Live-cell imaging of single receptor composition using zero-mode waveguide nanostructures.

Author information

1
Division of Biology 156-29, California Institute of Technology, 1200 East California Boulevard, Pasadena, California 91125, United States.

Abstract

We exploit the optical and spatial features of subwavelength nanostructures to examine individual receptors on the plasma membrane of living cells. Receptors were sequestered in portions of the membrane projected into zero-mode waveguides. Using single-step photobleaching of green fluorescent protein incorporated into individual subunits, the resulting spatial isolation was used to measure subunit stoichiometry in α4β4 and α4β2 nicotinic acetylcholine and P2X2 ATP receptors. We also show that nicotine and cytisine have differential effects on α4β2 stoichiometry.

PMID:
22668081
PMCID:
PMC3397148
DOI:
10.1021/nl301480h
[Indexed for MEDLINE]
Free PMC Article

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