Identification of proteins with potential osteogenic activity present in the water-soluble matrix proteins from Crassostrea gigas nacre using a proteomic approach

ScientificWorldJournal. 2012:2012:765909. doi: 10.1100/2012/765909. Epub 2012 May 1.

Abstract

Nacre, when implanted in vivo in bones of dogs, sheep, mice, and humans, induces a biological response that includes integration and osteogenic activity on the host tissue that seems to be activated by a set of proteins present in the nacre water-soluble matrix (WSM). We describe here an experimental approach that can accurately identify the proteins present in the WSM of shell mollusk nacre. Four proteins (three gigasin-2 isoforms and a cystatin A2) were for the first time identified in WSM of Crassostrea gigas nacre using 2DE and LC-MS/MS for protein identification. These proteins are thought to be involved in bone remodeling processes and could be responsible for the biocompatibility shown between bone and nacre grafts. These results represent a contribution to the study of shell biomineralization process and opens new perspectives for the development of new nacre biomaterials for orthopedic applications.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chromatography, Liquid
  • Crassostrea / metabolism*
  • Electrophoresis, Gel, Two-Dimensional
  • Osteogenesis / physiology*
  • Proteins / chemistry*
  • Proteins / physiology
  • Proteomics*
  • Solubility
  • Tandem Mass Spectrometry
  • Water / chemistry*

Substances

  • Proteins
  • Water