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ScientificWorldJournal. 2012;2012:765909. doi: 10.1100/2012/765909. Epub 2012 May 1.

Identification of proteins with potential osteogenic activity present in the water-soluble matrix proteins from Crassostrea gigas nacre using a proteomic approach.

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1
Center of Marine Sciences, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal.

Abstract

Nacre, when implanted in vivo in bones of dogs, sheep, mice, and humans, induces a biological response that includes integration and osteogenic activity on the host tissue that seems to be activated by a set of proteins present in the nacre water-soluble matrix (WSM). We describe here an experimental approach that can accurately identify the proteins present in the WSM of shell mollusk nacre. Four proteins (three gigasin-2 isoforms and a cystatin A2) were for the first time identified in WSM of Crassostrea gigas nacre using 2DE and LC-MS/MS for protein identification. These proteins are thought to be involved in bone remodeling processes and could be responsible for the biocompatibility shown between bone and nacre grafts. These results represent a contribution to the study of shell biomineralization process and opens new perspectives for the development of new nacre biomaterials for orthopedic applications.

PMID:
22666151
PMCID:
PMC3361287
DOI:
10.1100/2012/765909
[Indexed for MEDLINE]
Free PMC Article
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