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Biotechnol Lett. 2012 Sep;34(9):1765-71. doi: 10.1007/s10529-012-0959-7. Epub 2012 Jun 3.

Preparation and characterization of a VEGF-Fc fusion protein matrix for enhancing HUVEC growth.

Author information

1
School of Chemical Engineering, Tianjin University, Tianjin 300072, China. yumeihua19861230@yahoo.cn

Abstract

To enhance vascularization of hydrophobic implants in vivo, a VEGF-Fc fusion protein consisting of vascular endothelial growth factor (VEGF) fused to the immunoglobulin G Fc domain was prepared as an artificial extracellular matrix (ECM). VEGF-Fc was stably immobilized on a polystyrene plate due to the hydrophobicity of the Fc domain, and significantly enhanced the adhesion of human umbilical vein endothelial cells (HUVECs). Additionally, the use of VEGF-Fc as an ECM markedly promoted the proliferation of HUVECs longer than 72 h and induced the reorganization of actin filaments into larger stress fibers within these cells. The VEGF-Fc fusion protein may be a promising artificial ECM for enhancing endothelial cell growth.

PMID:
22661013
DOI:
10.1007/s10529-012-0959-7
[Indexed for MEDLINE]

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