Format

Send to

Choose Destination
Gene. 1990 Nov 30;96(1):29-36.

Genomic replacement in Escherichia coli K-12 using covalently closed circular plasmid DNA.

Author information

1
Department of Biochemistry, University of Illinois, Urbana 61801.

Abstract

A number of gene replacements at different loci were constructed using covalently closed circular (ccc) plasmid DNA in the recB21 recC22 sbcB15 sbcC201 mutant of Escherichia coli (JC7623). Selected constructs representing deletions and insertion mutations formed from double-crossover events involving the ccc plasmid molecules and the genome were confirmed by Southern blots, and the frequency of double-crossover events was evaluated. It is reported that such mutants may be constructed without linearizing plasmid DNA, as described previously.

PMID:
2265756
DOI:
10.1016/0378-1119(90)90337-q
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center