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DNA Cell Biol. 1990 Dec;9(10):777-81.

A simplified method for the preparation of transcriptionally active liver nuclear extracts.

Author information

1
Department of Medicine, University of California, San Diego, La Jolla 92093.

Abstract

We have developed a simplified method for the preparation of liver nuclear extracts to study gene regulation and protein-DNA interactions. This protocol uses conventional laboratory equipment and standard reagents. The liver tissue is homogenized in a low-salt solution at physiological molarity with subsequent adjustment of the molarity and purification of nuclei by density sedimentation. The nuclear extracts are transcriptionally active in a validated cell-free transcription assay and contain functional DNA-binding proteins. This protocol results in the rapid preparation of highly reproducible and active liver nuclear extracts.

PMID:
2264931
DOI:
10.1089/dna.1990.9.777
[Indexed for MEDLINE]

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