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ScientificWorldJournal. 2012;2012:372345. doi: 10.1100/2012/372345. Epub 2012 Apr 29.

Growth inhibition by caffeic acid, one of the phenolic constituents of honey, in HCT 15 colon cancer cells.

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1
Department of Biomedical Engineering, PSNA College of Engineering and Technology, Dindugal, Kothandaraman Nagar, Dindugal 624622, Tamil Nadu, India. jaganathaniitkgp@gmail.com

Abstract

Previous work from our laboratory showed that the mechanism of crude-honey induced apoptosis in colon cancer cells. Since phenolic constituents of honey were attributed to its apoptosis-inducing ability, we studied caffeic acid, one of the phenolic constituents of honey, induced effect on colon cancer cells. Antiproliferative effect of caffeic acid was estimated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. MTT assay signified the antiproliferative nature of caffeic acid against the HCT 15 colon cancer cells. A time-dependent inhibition of colony formation was evident with caffeic acid treatment. Cell-cycle analysis of caffeic acid- (CA-) treated cells indicated increasing accumulation of cells at sub-G(1) phase. Photomicrograph images of treated cells showed membrane blebbing and cell shrinkage. Yo-pro-1 staining of caffeic-acid-treated cells confirmed apoptosis in dose- and time-dependent manner. Increasing ROS generation and reduction in the mitochondrial membrane potential were also accompanied in the caffeic acid-induced apoptosis. This work will promote caffeic acid as a likely candidate in the chemoprevention of colon cancer.

PMID:
22649289
PMCID:
PMC3353276
DOI:
10.1100/2012/372345
[Indexed for MEDLINE]
Free PMC Article
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