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Arch Virol. 2012 Sep;157(9):1779-83. doi: 10.1007/s00705-012-1348-1. Epub 2012 May 30.

Development and evaluation of a reverse transcription loop-mediated isothermal amplification assay for rapid detection of a new SFTS bunyavirus.

Author information

1
Jinan Center for Disease Control and Prevention, Jinan, 250021 Shandong Province, China.

Abstract

The etiological agent of severe fever with thrombocytopenia syndrome (SFTS) is a bunyavirus that was first identified in China in 2009. We have developed and validated a one-step, single-tube, reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay for detection of SFTS bunyavirus (SFTSV). This assay demonstrated high specificity and sensitivity, with a detection limit of 10(1) TCID(50) ml(-1). When combined with the fluorescent detection reagent (FDR) method, results could be determined by observing a color change within 30 min. As an accurate, rapid, simple and low-cost diagnostic method, this RT-LAMP assay will be helpful for detecting and preventing further SFTSV infection in China.

PMID:
22643834
DOI:
10.1007/s00705-012-1348-1
[Indexed for MEDLINE]

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