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J Am Chem Soc. 2012 Jun 20;134(24):9918-21. doi: 10.1021/ja303904e. Epub 2012 Jun 6.

Synthesis of bispecific antibodies using genetically encoded unnatural amino acids.

Author information

1
Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.

Abstract

Bispecific antibodies were constructed using genetically encoded unnatural amino acids with orthogonal chemical reactivity. A two-step process afforded homogeneous products in excellent yield. Using this approach, we synthesized an anti-HER2/anti-CD3 bispecific antibody, which efficiently cross-linked HER2+ cells and CD3+ cells. In vitro effector-cell mediated cytotoxicity was observed at picomolar concentrations.

PMID:
22642368
PMCID:
PMC4299457
DOI:
10.1021/ja303904e
[Indexed for MEDLINE]
Free PMC Article

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