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Appl Environ Microbiol. 2012 Aug;78(15):5457-9. doi: 10.1128/AEM.00669-12. Epub 2012 May 25.

Reclamation of ampicillin sensitivity for the genetic manipulation of Legionella pneumophila.

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1
Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri, USA.

Abstract

Research on Legionella pneumophila, the causative agent of Legionnaires' disease, has been hampered due to the lack of selectable markers for genetic manipulation. We report the construction of a mutant strain of L. pneumophila lacking loxA, a chromosomally encoded β-lactamase, that has enhanced sensitivity to ampicillin. Also described are a method for converting Legionella strains to ampicillin sensitivity and conditions for utilizing bla as a selectable marker.

PMID:
22635996
PMCID:
PMC3416426
DOI:
10.1128/AEM.00669-12
[Indexed for MEDLINE]
Free PMC Article
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