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J Phys Chem B. 2012 Jun 21;116(24):7162-7. doi: 10.1021/jp303455w. Epub 2012 Jun 8.

Measurements of 13C multiple-quantum coherences in amyloid fibrils under magic-angle spinning.

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1
Department of Chemistry, National Taiwan University, No. 1, Section 4, Roosevelt Road, Taipei, Taiwan.

Abstract

The excitation and detection of high-order multiple quantum coherences among (13)C nuclear spins are demonstrated in the samples of [1-(13)C]-L-alanine and (13)C labeled amyloid fibrils at a spinning frequency of 20 kHz. The technique is based on the double-quantum average Hamiltonian prepared by the DRAMA-XY4 pulse sequence. Empirically, we find that multiple supercycles are required to suppress the higher-order effects for real applications. Measurements for the fibril samples formed by the polypeptides of PrP(113-127) provide the first solid-state NMR evidence for the stacking of multiple β-sheet layers at the structural core of amyloid fibrils.

PMID:
22632418
DOI:
10.1021/jp303455w
[Indexed for MEDLINE]
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