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Biochemistry. 2012 Jun 12;51(23):4735-42. doi: 10.1021/bi3004022. Epub 2012 Jun 4.

Human complement C3 is a substrate for transglutaminases. A functional link between non-protease-based members of the coagulation and complement cascades.

Author information

1
Department of Molecular Biology and Genetics, University of Aarhus, Aarhus C, Denmark.

Abstract

In this study, we report the finding of functional cross-talk between two non-protease components of the complement and coagulation cascades. We show that complement C3, a central component of the complement system, is associated with the fibrin clot and that C3 becomes covalently cross-linked to other proteins during coagulation. Enzymatic incorporation of dansylcadaverine and dansyl-PGGQQIV into C3 by coagulation factor XIIIa and tissue transglutaminase demonstrated that C3 is a transglutaminase substrate. This suggested that coagulation factor XIIIa covalently cross-links C3 to clot components during coagulation. Using mass spectrometry, we verified that C3 indeed is covalently associated with the fibrin clot in a ratio of 0.05:1 relative to the known coagulation factor XIIIa substrate α2-antiplasmin.

PMID:
22630814
DOI:
10.1021/bi3004022
[Indexed for MEDLINE]

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