(A–C) Survival of mice orthotopically transplanted with 100,000 NSCs isolated from forebrain, cerebellum and brain stem/LRL, transduced with N-mycWT or N-mycT58A viruses. n=number of mice per group. Mice injected with N-mycWT NSCs (green colors) or N-mycT58A E16 and P0 brain stem/LRL (red and green, respectively) generated no tumors.
(D–I) Histopathology of representative P0 cerebellar (D and E), P0 forebrain (F and G), and E14 brain stem/LRL (H–I) N-mycT58A tumors (H&E staining). Cerebellar tumors displayed MB features, including cell wrapping in a tumor with prominent LC/A features (D, arrowhead) and Homer Wright rosettes (E, arrowhead). Forebrain tumors displayed diffuse invasion of tumor cells along white matter tracts (arrowhead) (G). LRL tumors displayed marked cellular and nuclear pleomorphism (I, arrowhead). Box in H (200x) denotes region shown at higher magnification in I (400x).
(J–U) Immunostaining, of representative N-mycT58A-induced cerebellar, forebrain, and LRL tumors demonstrating greater neuronal differentiation in cerebellar tumors, greater glial differentiation in forebrain tumors, and intermediate differentiation in LRL tumors. * indicate tumor region in pictures where an adjacent normal brain is present.
Scale: 100μm. See also and .