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FEBS Lett. 1990 Nov 26;275(1-2):177-80.

K-252a inhibits the response of tomato cells to fungal elicitors in vivo and their microsomal protein kinase in vitro.

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1
Friedrich Miescher-Institute, Basel, Switzerland.

Abstract

Two characteristic responses of plant cells to fungal elicitors, induction of phenylalanine ammonia-lyase activity and of ethylene biosynthesis, were studied in suspension-cultured tomato cells. Induction of both responses was completely blocked by 500 nM K-252a, a known inhibitor of mammalian protein kinases. About 100 nM K-252a caused half-maximal inhibition. In vitro, K-252a inhibited protein kinase activity in microsomal preparations from tomato cells. Inhibition was competitive with respect to ATP and had a Ki of about 15 nM. Thus, protein kinases sensitive to K-252a occur in plants and might be important for the plant's response to fungal elicitors.

PMID:
2261987
DOI:
10.1016/0014-5793(90)81466-2
[Indexed for MEDLINE]
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