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Genes Chromosomes Cancer. 2012 Sep;51(9):868-80. doi: 10.1002/gcc.21971. Epub 2012 May 23.

GAS41 amplification results in overexpression of a new spindle pole protein.

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1
Department of Human Genetics, Saarland University, Medical School, Homburg, Germany. jana.schmitt@uks.eu

Abstract

Amplification is a hallmark of many human tumors but the role of most amplified genes in human tumor development is not yet understood. Previously, we identified a frequently amplified gene in glioma termed glioma-amplified sequence 41 (GAS41). Using the TCGA data portal and performing experiments on HeLa and TX3868, we analyzed the role of GAS41 amplification on GAS41 overexpression and the effect on the cell cycle. Here we show that GAS41 amplification is associated with overexpression in the majority of cases. Both induced and endogenous overexpression of GAS41 leads to an increase in multipolar spindles. We showed that GAS41 is specifically associated with pericentrosome material. As result of an increased GAS41 expression we found bipolar spindles with misaligned chromosomes. This number was even increased by a combined overexpression of GAS41 and a reduced expression of NuMA. We propose that GAS41 amplification may have an effect on the highly altered karyotype of glioblastoma via its role during spindle pole formation.

PMID:
22619067
PMCID:
PMC4476279
DOI:
10.1002/gcc.21971
[Indexed for MEDLINE]
Free PMC Article
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