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Chem Cent J. 2012 May 22;6(1):43. doi: 10.1186/1752-153X-6-43.

Assessment of flavonoids contents and in vitro antioxidant activity of Launaea procumbens.

Author information

1
Department of Biotechnology, Faculty of Biological Sciences, University of Science and Technology, Bannu, KPK, 28100, Pakistan. Rahmatgul_81@yahoo.com.

Abstract

BACKGROUND:

Launaea procumbens (LP) has been used as a food supplement in Pakistan. In this study methanolic crude extract (LPME) of the whole plant and its different fractions; n-hexane (LPHE); ethyl acetate (LPEE) and chloroform (LPCE) were studied for the determination of total flavonoid and phenolics contents along with multifaceted in vitro scavenging assays.

RESULTS:

Considerable amount of flavonoid and phenolics contents were found in all the fractions. Methanol and chloroform fraction exhibited efficient scavenging of DPPH·, ABTS·+, ·OH, superoxide, lipid peroxide and nitric oxide free radicals. Significant correlation was found between DPPH·, ABTS·+, superoxide radical, β-carotene bleaching restraint and phosphomolybdenum assay with total flavonoids and phenolics contents. High performance chromatography (HPLC) of LPME revealed the presence of vitexin, orientin, rutin, hyperoside, catechin and myricetin.

CONCLUSION:

These results reveal the presence of bioactive compounds in LPME, which might be contributed towards the various in vitro scavenging.

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