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Appl Opt. 2012 May 20;51(15):2842-6. doi: 10.1364/AO.51.002842.

Use of attenuated total reflectance Fourier transform infrared spectroscopy to monitor the development of lipid aggregate structures.

Author information

1
Department of Chemistry, University of California-Davis, Davis, California 95616, USA.

Abstract

Attenuated total reflectance Fourier transform infrared spectroscopy is used to monitor the adsorption of 100 nm 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) phospholipid vesicles to the surfaces of Ge, electrolessly deposited Au, and a well formed self-assembled monolayer of 1-octadecanethiol. The interaction of DPPC vesicles in solution with these different surfaces yields distinctly different surface structures: intact DPPC vesicles on Ge, a supported phospholipid bilayer on an electrolessly deposited Au surface, and a phospholipid monolayer onto the hydrophobic self-assembled monolayer. IR peak position, bandwidth, and intensity are used to confirm structure formation and quantitation of the amount of lipid that desorbs during film formation.

PMID:
22614584
[Indexed for MEDLINE]

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