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J Cancer Res Clin Oncol. 2012 Oct;138(10):1619-24. Epub 2012 May 22.

CD133 expression in renal cell carcinoma (RCC) is correlated with nuclear hypoxia-inducing factor 1α (HIF-1α).

Author information

1
The Department of Urology, The Second Affiliated Hospital of Soochow University, Suzhou 215006, China.

Abstract

PURPOSE:

In our previous study, we have found that the hypoxia-inducible factor 1α (HIF-1α) is upregulated in renal cell carcinoma (RCC) tissues compared with para-cancer normal tissues by 2-dimensional polyacrylamide gel electrophoresis. It was reported that hypoxic conditions were correlated with cancer stem cell generation and HIF-1α acted as a transcription regulator in nuclear HIF-1α expression. Therefore, in this study we investigate the relation between CD133 and nuclear HIF-1α expression levels in RCC tissues.

METHODS:

In this study 61 RCC tissues from the patients that treated with radical nephrectomy were collected. Then, we investigated the expression of CD133 and nuclear HIF-1α expression by immunohistochemistry. To verify the relation between CD133 and nuclear HIF-1α expression, we treated 786-O cells with cobalt chloride. The expression of CD133 on 786-O cells was analyzed by flowcytometry.

RESULTS:

The immunohistochemical study showed that CD133 was correlated with tumor stage and metastatic stage, whereas nuclear HIF-1α had no association with clinicopathological parameters. However, the expression of nuclear HIF-1α was correlated with CD133. The CD133 expression in 786-O cells was enhanced by cobalt chloride, which meant that CD133 expression was affected by hypoxia.

CONCLUSIONS:

Our study showed that in RCC, CD133 expression was strongly related to nuclear HIF-1α and the expression of CD133 might be upregulated under hypoxia environment.

PMID:
22614155
DOI:
10.1007/s00432-012-1237-8
[Indexed for MEDLINE]
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