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Bioorg Med Chem Lett. 2012 Jun 15;22(12):3870-2. doi: 10.1016/j.bmcl.2012.04.123. Epub 2012 May 4.

Production of truncated protein by the crosslink formation of mRNA with 2'-OMe oligoribonucleotide containing 2-amino-6-vinylpurine.

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1
Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, 2-1-1 Katahira, Miyagi 980-8577, Japan.

Abstract

The development of convenient methods for controlling the protein expression is an important challenge in the postgenomic era. We applied the crosslink forming oligonucleotide (CFO) as a terminator of the ribosomal translation. In this study, we demonstrated that the improved reactivity of our CFO under physiological conditions enabled the sequence-specific introduction of a steric block for a ribosome on mRNAs. In vitro and in cell translation experiments revealed that the crosslinked mRNA can produce the truncated proteins in which the translation terminates at the desired position.

PMID:
22613261
DOI:
10.1016/j.bmcl.2012.04.123
[Indexed for MEDLINE]
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