Format

Send to

Choose Destination
See comment in PubMed Commons below
Chimia (Aarau). 2012;66(4):159-65. doi: 10.2533/chimia.2012.159.

Molecular rotors image intracellular viscosity.

Author information

  • 1Chemistry Department, Imperial College, South Kensington SW7 2AZ, London, UK. m.kuimova@imperial.ac.uk

Abstract

This review describes a new method for quantitative measurement and spatial imaging of microviscosity within individual domains of live cells. The method is based on fluorescence detection from small synthetic molecules termed 'molecular rotors', which are characterised by a strong response of fluorescence lifetimes or spectra to the viscosity of their immediate environment. We have demonstrated that the quantitative determination of viscosity is possible using lifetime-based molecular rotors and ratiometric molecular rotors. The ratiometric imaging of viscosity benefits from a very fast signal acquisition. We have illustrated this advantage by monitoring changes in intracellular viscosity during photodynamic therapy, which is a clinically utilised modality for the treatment of neoplastic disease.

PMID:
22613140
DOI:
10.2533/chimia.2012.159
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Ingenta plc
    Loading ...
    Support Center