Send to

Choose Destination
J Biomed Opt. 2012 May;17(5):056004. doi: 10.1117/1.JBO.17.5.056004.

Optimal ultraviolet wavelength for in vivo photoacoustic imaging of cell nuclei.

Author information

Washington University in St. Louis, Department of Biomedical Engineering, St. Louis, MO 63130, USA.


In order to image noninvasively cell nuclei in vivo without staining, we have developed ultraviolet photoacoustic microscopy (UV-PAM), in which ultraviolet light excites nucleic acids in cell nuclei to produce photoacoustic waves. Equipped with a tunable laser system, the UV-PAM was applied to in vivo imaging of cell nuclei in small animals. We found that 250 nm was the optimal wavelength for in vivo photoacoustic imaging of cell nuclei. The optimal wavelength enables UV-PAM to image cell nuclei using as little as 2 nJ laser pulse energy. Besides the optimal wavelength, application of a wavelength between 245 and 275 nm can produce in vivo images of cell nuclei with specific, positive, and high optical contrast.

[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Society of Photo-Optical Instrumentation Engineers Icon for PubMed Central
Loading ...
Support Center