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Methods Mol Biol. 2012;877:325-41. doi: 10.1007/978-1-61779-818-4_25.

Basic procedures for epigenetic analysis in plant cell and tissue culture.

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Área de Fisiología Vegetal, Departamento BOS, Facultad de Biología, Universidad de Oviedo, Oviedo, Spain.


In vitro culture is one of the most studied techniques, and it is used to study many developmental processes, especially in forestry species, because of growth timing and easy manipulation. Epigenetics has been shown as an important influence on many research analyses such as cancer in mammals and developmental processes in plants such as flowering, but regarding in vitro culture, techniques to study DNA methylation or chromatin modifications were mainly limited to identify somaclonal variation of the micropropagated material. Because in vitro culture is not only a way to generate plant material but also a bunch of differentially induced developmental processes, an approach of techniques and some research carried out to study the different changes regarding DNA methylation and chromatin and translational modifications that take place during these processes is reviewed.

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