Abstract
We developed one-step sequence- and ligation-independent cloning (SLIC) as a simple, cost-effective, time-saving, and versatile cloning method. Highly efficient and directional cloning can be achieved by direct bacterial transformation 2.5 min after mixing any linearized vector, an insert(s) prepared by PCR, and T4 DNA polymerase in a tube at room temperature.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Cloning, Molecular / methods*
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DNA-Directed DNA Polymerase / metabolism
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Genetic Vectors / genetics
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Genomics / methods*
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Molecular Sequence Data
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Polymerase Chain Reaction
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Transformation, Bacterial / genetics*
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Viral Proteins / metabolism
Substances
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Viral Proteins
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gene 43 protein, Enterobacteria phage T4
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DNA-Directed DNA Polymerase