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Appl Environ Microbiol. 2012 Aug;78(15):5432-4. doi: 10.1128/AEM.01310-12. Epub 2012 May 18.

A gain-of-function mutation in gating of Corynebacterium glutamicum NCgl1221 causes constitutive glutamate secretion.

Author information

1
Department of Biology, Tokyo Gakugei University, Koganei-shi, Tokyo, Japan. y_nakayama9339@nifty.com

Abstract

The A-to-V mutation at position 111 (A111V) in the mechanosensitive channel NCgl1221 (MscCG) causes constitutive glutamate secretion in Corynebacterium glutamicum. Patch clamp experiments revealed that NCgl1221 (A111V) had a significantly smaller gating threshold than the wild-type counterpart and displayed strong hysteresis, suggesting that the gain-of-function mutation in the gating of NCgl1221 leads to the oversecretion of glutamate.

PMID:
22610427
PMCID:
PMC3416442
DOI:
10.1128/AEM.01310-12
[Indexed for MEDLINE]
Free PMC Article
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