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Nat Methods. 2012 May 20;9(8):808-10. doi: 10.1038/nmeth.2023.

2b-RAD: a simple and flexible method for genome-wide genotyping.

Author information

1
School of Biological Sciences, University of Texas at Austin, Austin, Texas, USA. swang@ouc.edu.cn

Abstract

We describe 2b-RAD, a streamlined restriction site-associated DNA (RAD) genotyping method based on sequencing the uniform fragments produced by type IIB restriction endonucleases. Well-studied accessions of Arabidopsis thaliana were genotyped to validate the method's accuracy and to demonstrate fine-tuning of marker density as needed. The simplicity of the 2b-RAD protocol makes it particularly suitable for high-throughput genotyping as required for linkage mapping and profiling genetic variation in natural populations.

PMID:
22609625
DOI:
10.1038/nmeth.2023
[Indexed for MEDLINE]

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