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Methods Enzymol. 2012;510:317-30. doi: 10.1016/B978-0-12-415931-0.00017-3.

Transformation of Clostridium thermocellum by electroporation.

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1
Thayer School of Engineering at Dartmouth College, Hanover, New Hampshire, USA.

Abstract

In this work, we provide detailed instructions for transformation of Clostridium thermocellum by electroporation. In addition, we describe two schemes for genetic modification: allelic replacement-where the gene of interest is replaced by an antibiotic marker and markerless gene deletion-where the gene of interest is removed and the selective markers are recycled. The markerless gene deletion technique can also be used for insertion of genes onto the C. thermocellum chromosome.

[Indexed for MEDLINE]

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