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J Biosci Bioeng. 2012 Jul;114(1):23-7. doi: 10.1016/j.jbiosc.2012.02.014. Epub 2012 May 17.

Indolepyruvate ferredoxin oxidoreductase: An oxygen-sensitive iron-sulfur enzyme from the hyperthermophilic archaeon Thermococcus profundus.

Author information

1
Department of Life Science and Graduate School of Life Science, Rikkyo (St. Paul's) University, Toshima-ku, Tokyo 171-8501, Japan.

Abstract

Thermococcus profundus is a strictly anaerobic sulfur-dependent archaeon that grows optimally at 80°C by peptide fermentation. Indolepyruvate ferredoxin oxidoreductase (IOR), an enzyme involved in the peptide fermentation pathway, was purified to homogeneity from the archaeon under strictly anaerobic conditions. The maximal activity was obtained above the boiling temperature of water (105°C), with a half-life of 62min at 100°C and 20min at 105°C. IOR was oxygen-sensitive with a half-life of 7h at 25°C under aerobic conditions. The specific activity of T. profundus IOR was found to be dependent on the number of [4Fe-4S] clusters in the enzyme.

PMID:
22608551
DOI:
10.1016/j.jbiosc.2012.02.014
[Indexed for MEDLINE]

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