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J Clin Virol. 2012 Jul;54(3):240-4. doi: 10.1016/j.jcv.2012.04.007. Epub 2012 May 15.

Human cytomegalovirus preferentially infects the neoplastic epithelium of colorectal cancer: a quantitative and histological analysis.

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1
Department of Medicine, National Yang-Ming University Hospital, Yilan, Taiwan.

Abstract

BACKGROUND:

It has long been suggested that human cytomegalovirus (HCMV) might be involved in human oncogenesis. However, whether HCMV was associated with colorectal cancer (CRC) was still controversial.

OBJECTIVE:

To clarify whether HCMV specifically infects the tumorous tissue of CRC.

STUDY DESIGN:

Paired tumor and adjacent non-neoplastic CRC specimens were collected from 163 patients. HCMV DNA was detected and quantified through PCR and quantitative real-time PCR. Virus location was determined by in situ hybridization (ISH) of formalin-fixed paraffin-embedded tissue sections with an HCMV-specific probe.

RESULTS:

By PCR, HCMV DNA was detected in 42.3% (69/163) of the tumor specimens, while only 5.6%(14/163) samples of adjacent non-neoplastic tissue were positive for HCMV (p<0.0001). Quantitative real-time PCR in 54 sample pairs revealed significantly higher viral copies in the tumor specimens than the adjacent non-neoplastic tissue specimens (p<0.001). By ISH, the nucleic acids of HCMV were detected in the cytoplasm of neoplastic epithelium. No hybridization was detected in the inflammatory infiltrates, submucosa, or other stromal tissues.

CONCLUSIONS:

HCMV preferentially infects the tumor epithelium of CRC. How the virus subsists in and interacts with the microenvironment of tumor epithelium of CRC should be studied.

PMID:
22595308
DOI:
10.1016/j.jcv.2012.04.007
[Indexed for MEDLINE]

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